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osm  (R&D Systems)


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    Structured Review

    R&D Systems osm
    Osm, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+osm/Recombinant+Human+Oncostatin+M+(OSM)+Protein/pm41986711-647-160-164
    Average 95 stars, based on 144 article reviews
    osm - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Oncostatin M suppresses IL31RA expression in dorsal root ganglia and interleukin-31-induced itching
    Article Snippet: Cells were stimulated with recombinant human OSM (100 ng/mL, R&D Systems) for 1, 3, 6, and 24 h, and gene expression changes analyzed by RT-qPCR (Section 2.9). .. In addition, cells were stimulated with various concentrations of recombinant human OSM (0.1–100 ng/mL, R&D Systems) for 3 h. and gene expression changes were analyzed by RT-qPCR (Section 2.9). .. Male C57BL/6 mice aged 8 to 12 weeks were euthanized with carbon dioxide and DRGs promptly dissected out and cultured in Dulbecco’s modified Eagle’s medium (Gibco ThermoFisher) supplemented with 10% fetal bovine serum (Gibco ThermoFisher) and penicillin/streptomycin at 37°C under a humidified atmosphere of 5% CO 2 .

    Article Title: Widely targeted metabolomics analysis of different parts of kudzu.
    Article Snippet: Breast cancer cells frequently home to the bone marrow, where they encounter signals that promote survival and quiescence or stimulate their proliferation.. The interleukin-6 (IL-6) cytokines signal through the co-receptor glycoprotein130 (gp130) and are abundantly secreted within the bone microenvironment.. Breast cancer cell expression of leukemia inhibitory factor (LIF) receptor (LIFR)/ STAT3 signaling promotes tumor dormancy in the bone, but it is unclear which, if any of the cytokines that signal through LIFR, including LIF, oncostatin M (OSM), and ciliary neurotrophic factor (CNTF), promote tumor dormancy and which signaling pathways are induced.

    Article Title: Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures
    Article Snippet: .. After 24 h incubation, the cells were exposed to either 25 mM Gadavist or regular KSFM (control) for 3 h. Subsequently, the wells were rinsed, and cells were exposed to recombinant human OSM (0.1 and 1 ng mL –1 ; R&D Systems) and IL6 (0.1 and 1 ng mL –1 ; R&D Systems) for 12 h. The pSTAT3 levels were measured in the HBEC3 KT lysates using the pSTAT3 (Y705) DuoSet ELISA kit (R&D Systems), as per the manufacturer’s instructions. ..

    Article Title: Oncostatin M suppresses IL31RA expression in dorsal root ganglia and interleukin-31-induced itching
    Article Snippet: The keratinocyte cell line Human Epidermal Keratinocytes, pooled (HEK) was obtained from Gibco (Gibco ThermoFisher, NY, USA) and cultured in Keratinocyte SFM (Gibco ThermoFisher) supplemented with 5 μg/mL human recombinant epidermal growth factor (Gibco ThermoFisher), 50 μg/mL bovine pituitary extract (Gibco ThermoFisher), and 100 IU/mL penicillin/streptomycin (Gibco ThermoFisher). .. Cells were stimulated with recombinant human OSM (100 ng/mL, R&D Systems) for 1, 3, 6, and 24 h, and gene expression changes analyzed by RT-qPCR (Section 2.9). .. In addition, cells were stimulated with various concentrations of recombinant human OSM (0.1–100 ng/mL, R&D Systems) for 3 h. and gene expression changes were analyzed by RT-qPCR (Section 2.9).

    Gene Expression:

    Article Title: Oncostatin M suppresses IL31RA expression in dorsal root ganglia and interleukin-31-induced itching
    Article Snippet: Cells were stimulated with recombinant human OSM (100 ng/mL, R&D Systems) for 1, 3, 6, and 24 h, and gene expression changes analyzed by RT-qPCR (Section 2.9). .. In addition, cells were stimulated with various concentrations of recombinant human OSM (0.1–100 ng/mL, R&D Systems) for 3 h. and gene expression changes were analyzed by RT-qPCR (Section 2.9). .. Male C57BL/6 mice aged 8 to 12 weeks were euthanized with carbon dioxide and DRGs promptly dissected out and cultured in Dulbecco’s modified Eagle’s medium (Gibco ThermoFisher) supplemented with 10% fetal bovine serum (Gibco ThermoFisher) and penicillin/streptomycin at 37°C under a humidified atmosphere of 5% CO 2 .

    Article Title: Oncostatin M suppresses IL31RA expression in dorsal root ganglia and interleukin-31-induced itching
    Article Snippet: The keratinocyte cell line Human Epidermal Keratinocytes, pooled (HEK) was obtained from Gibco (Gibco ThermoFisher, NY, USA) and cultured in Keratinocyte SFM (Gibco ThermoFisher) supplemented with 5 μg/mL human recombinant epidermal growth factor (Gibco ThermoFisher), 50 μg/mL bovine pituitary extract (Gibco ThermoFisher), and 100 IU/mL penicillin/streptomycin (Gibco ThermoFisher). .. Cells were stimulated with recombinant human OSM (100 ng/mL, R&D Systems) for 1, 3, 6, and 24 h, and gene expression changes analyzed by RT-qPCR (Section 2.9). .. In addition, cells were stimulated with various concentrations of recombinant human OSM (0.1–100 ng/mL, R&D Systems) for 3 h. and gene expression changes were analyzed by RT-qPCR (Section 2.9).

    Quantitative RT-PCR:

    Article Title: Oncostatin M suppresses IL31RA expression in dorsal root ganglia and interleukin-31-induced itching
    Article Snippet: Cells were stimulated with recombinant human OSM (100 ng/mL, R&D Systems) for 1, 3, 6, and 24 h, and gene expression changes analyzed by RT-qPCR (Section 2.9). .. In addition, cells were stimulated with various concentrations of recombinant human OSM (0.1–100 ng/mL, R&D Systems) for 3 h. and gene expression changes were analyzed by RT-qPCR (Section 2.9). .. Male C57BL/6 mice aged 8 to 12 weeks were euthanized with carbon dioxide and DRGs promptly dissected out and cultured in Dulbecco’s modified Eagle’s medium (Gibco ThermoFisher) supplemented with 10% fetal bovine serum (Gibco ThermoFisher) and penicillin/streptomycin at 37°C under a humidified atmosphere of 5% CO 2 .

    Article Title: Oncostatin M suppresses IL31RA expression in dorsal root ganglia and interleukin-31-induced itching
    Article Snippet: The keratinocyte cell line Human Epidermal Keratinocytes, pooled (HEK) was obtained from Gibco (Gibco ThermoFisher, NY, USA) and cultured in Keratinocyte SFM (Gibco ThermoFisher) supplemented with 5 μg/mL human recombinant epidermal growth factor (Gibco ThermoFisher), 50 μg/mL bovine pituitary extract (Gibco ThermoFisher), and 100 IU/mL penicillin/streptomycin (Gibco ThermoFisher). .. Cells were stimulated with recombinant human OSM (100 ng/mL, R&D Systems) for 1, 3, 6, and 24 h, and gene expression changes analyzed by RT-qPCR (Section 2.9). .. In addition, cells were stimulated with various concentrations of recombinant human OSM (0.1–100 ng/mL, R&D Systems) for 3 h. and gene expression changes were analyzed by RT-qPCR (Section 2.9).

    Control:

    Article Title: Bispecific antigen binding molecules that bind leptin receptor and/or GP130, and methods of use thereof
    Article Snippet: The isotype control and human OSM (hOSM; R&D Systems, #295-OM/CF) were half-log diluted (11 points) to final concentrations ranging from 100 nM to 1 pM in Assay Buffer and were then added to the cells. .. The isotype control and human OSM (hOSM; R&D Systems, #295-OM/CF) were half-log diluted (11 points) to final concentrations ranging from 100 nM to 1 pM in Assay Buffer and were then added to the cells. .. One-Glo reagent (Promega, #E6051) was then added to the samples and luciferase activity was measured on Envision Multilable Plate Reader (Perkin Elmer) in Luminescent mode.

    Article Title: Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures
    Article Snippet: .. After 24 h incubation, the cells were exposed to either 25 mM Gadavist or regular KSFM (control) for 3 h. Subsequently, the wells were rinsed, and cells were exposed to recombinant human OSM (0.1 and 1 ng mL –1 ; R&D Systems) and IL6 (0.1 and 1 ng mL –1 ; R&D Systems) for 12 h. The pSTAT3 levels were measured in the HBEC3 KT lysates using the pSTAT3 (Y705) DuoSet ELISA kit (R&D Systems), as per the manufacturer’s instructions. ..

    Incubation:

    Article Title: Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures
    Article Snippet: .. After 24 h incubation, the cells were exposed to either 25 mM Gadavist or regular KSFM (control) for 3 h. Subsequently, the wells were rinsed, and cells were exposed to recombinant human OSM (0.1 and 1 ng mL –1 ; R&D Systems) and IL6 (0.1 and 1 ng mL –1 ; R&D Systems) for 12 h. The pSTAT3 levels were measured in the HBEC3 KT lysates using the pSTAT3 (Y705) DuoSet ELISA kit (R&D Systems), as per the manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures
    Article Snippet: .. After 24 h incubation, the cells were exposed to either 25 mM Gadavist or regular KSFM (control) for 3 h. Subsequently, the wells were rinsed, and cells were exposed to recombinant human OSM (0.1 and 1 ng mL –1 ; R&D Systems) and IL6 (0.1 and 1 ng mL –1 ; R&D Systems) for 12 h. The pSTAT3 levels were measured in the HBEC3 KT lysates using the pSTAT3 (Y705) DuoSet ELISA kit (R&D Systems), as per the manufacturer’s instructions. ..

    Article Title: Oncostatin M for characterizing the inflammatory burden and outcome of V-V ECMO in ARDS patients.
    Article Snippet: Venovenous extracorporeal membrane oxygenation (VV ECMO) has become increasingly important for supporting patients with refractory acute respiratory failure.1 Plasma lactate (pLA), a laboratory marker for tissue hypoxia and hypoperfusion,2 has been widely used to assess the anticipated outcome of ECMO support despite uncertainties about its reliability.3,4 However, potential markers that are more directly associated with the pathogenesis of acute respiratory failure itself have not been thoroughly investigated.. We hypothesized that plasma oncostatin M Received: 27 February 2023 | Revised: 11 July 2023 | Accepted: 14 July 2023 DOI: 10.1111/aor.14619

    Clinical Proteomics:

    Article Title: Oncostatin M for characterizing the inflammatory burden and outcome of V-V ECMO in ARDS patients.
    Article Snippet: Venovenous extracorporeal membrane oxygenation (VV ECMO) has become increasingly important for supporting patients with refractory acute respiratory failure.1 Plasma lactate (pLA), a laboratory marker for tissue hypoxia and hypoperfusion,2 has been widely used to assess the anticipated outcome of ECMO support despite uncertainties about its reliability.3,4 However, potential markers that are more directly associated with the pathogenesis of acute respiratory failure itself have not been thoroughly investigated.. We hypothesized that plasma oncostatin M Received: 27 February 2023 | Revised: 11 July 2023 | Accepted: 14 July 2023 DOI: 10.1111/aor.14619



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